[Cloning and expression of gB gene of infectious laryngotracheitis virus in M. smegmatis].

ABSTRACT

Firstly, the complete gB gene of a domestic isolation stain were amplified by PCR, and the 2.6 kb gene fragment was obtained. Then the recombinant plasmid pY-alpha-gB was constructed by cloning PCR product into pY-alpha vector, and the shuttle expression plasmid pR-alpha-gB was constructed by cloning the hsp-alpha-gB gene into the downstream sequences of pRR3 vector. The recombinant plasmid was identified by restriction enzyme digestion and the sequence analysis, which was electrophoreted into M. smegmatis mc2 155. At last, the expressed gB proteins were successfully detected by ELISA and Western blot, which seems to be immunogenic crucially. The recombinant bacterial stain M. smegmatis mc2 155 (pR-alpha-gB)could protect SPF chick embryo from one lethal dose of ILTV.