Utilization of a zebra finch BAC library to determine the structure of an avian androgen receptor genomic region.
Genomics
; 87(1): 181-90, 2006 Jan.
Article
em En
| MEDLINE
| ID: mdl-16321505
ABSTRACT
The zebra finch (Taeniopygia guttata) is an important model organism for studying behavior, neuroscience, avian biology, and evolution. To support the study of its genome, we constructed a BAC library (TG__Ba) using DNA from livers of females. The BAC library consists of 147,456 clones with 98% containing inserts of an average size of 134 kb and represents 15.5 haploid genome equivalents. By sequencing a whole BAC, a full-length androgen receptor open reading frame was identified, the first in an avian species. Comparison of BAC end sequences and the whole BAC sequence with the chicken genome draft sequence showed a high degree of conserved synteny between the zebra finch and the chicken genome.
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Base de dados:
MEDLINE
Assunto principal:
Receptores Androgênicos
/
Biblioteca Genômica
/
Fases de Leitura Aberta
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Cromossomos Artificiais Bacterianos
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Tentilhões
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Estados Unidos