Characterization of Toxoplasma gondii isolates in free-range chickens from Amazon, Brazil.
J Parasitol
; 92(1): 36-40, 2006 Feb.
Article
em En
| MEDLINE
| ID: mdl-16629312
ABSTRACT
The prevalence of Toxoplasma gondii in free-ranging chickens is a good indicator of the prevalence of T. gondii oocysts in the soil because chickens feed from the ground. The prevalence of T. gondii in 50 free-range chickens (Gallus domesticus) from Amazon, Brazil, was determined. Antibodies to T. gondii were assayed by the modified agglutination test (MAT) and found in 33 (66%) chickens with titers of 15 in 3, 110 in 2, 120 in 1, 140 in 1, 180 in 2, 1160 in 5, 1200 in 9, 1400 in 5, 1800 in 2, 11,600 in 2, and 13,200 or higher in 1. Hearts and brains of 33 seropositive chickens were bioassayed individually in mice. Tissues from 17 seronegative chickens were pooled and fed to 2 T. gondii-free cats. Feces of cats were examined for oocysts, but none was found. Toxoplasma gondii was isolated from 24 chickens with MAT titers of 15 or higher. Genotyping of these 24 T. gondii isolates by polymorphisms at the SAG2 locus indicated that 14 were type I, and 10 were type III; the absence of type II strains from Brazil was confirmed. Fifty percent of the infected mice died of toxoplasmosis, irrespective of the genotype.
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Base de dados:
MEDLINE
Assunto principal:
Doenças das Aves Domésticas
/
Toxoplasma
/
Galinhas
/
Toxoplasmose Animal
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Estados Unidos