High dead volume pumps: delay time reduction by short-term low-ratio split flow in LC/MS applications.
J Am Soc Mass Spectrom
; 18(2): 245-7, 2007 Feb.
Article
em En
| MEDLINE
| ID: mdl-17095244
ABSTRACT
The aim of our work was to develop a low-cost, simple and reliable solution to reduce LC/MS analysis time by compensating for limitations inherent to high dead volume standard HPLC pumps. In our approach, we utilized a temporary (sub-1 min) low ratio flow split (approximately 110) at 5 mL/min pump flow before the column. During this short period, 90% of the entire pump flow is delivered to waste and used for fast pump/system equilibration. Although full-time flow splitting is widely used in capillary/nano applications (usually with high split ratios in the hundreds or thousands), to our knowledge, this is the first time that short-term low-ratio flow splitting has been used in conventional LC/MS applications.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
/
Centrifugação com Gradiente de Concentração
/
Cromatografia Líquida de Alta Pressão
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos