Regulation and function of collagenous repeat containing sequence of 26-kDa protein gene product "cartonectin".
Obesity (Silver Spring)
; 15(2): 303-13, 2007 Feb.
Article
em En
| MEDLINE
| ID: mdl-17299102
ABSTRACT
OBJECTIVE:
Collagenous repeat containing sequence of 26-kDa protein (CORS-26) was identified as a new gene transcript expressed in cartilage with unknown function. It was the aim of this study to investigate expression, regulation, and function of CORS-26 in adipocytes. RESEARCH METHODS AND PROCEDURES CORS-26 mRNA and protein expression was studied by reverse transcriptase-polymerase chain reaction, Western blot analysis, and quantitative real-time polymerase chain reaction. Transcriptional regulation was studied by electrophoretic mobility shift assay and luciferase reporter gene assay. The adipocytic secretion of adiponectin and resistin was measured by enzyme-linked immunosorbent assay.RESULTS:
CORS-26 mRNA is absent in 3T3-L1 preadipocytes and adipocytes after 48 hours of differentiation. CORS-26 mRNA was induced from Day 4 to Day 9 of adipocyte differentiation. CORS-26 protein was induced in mature adipocytes. Peroxisome proliferator-activated receptor (PPAR) gamma (but not PPARalpha) in nuclear extracts prepared from adipocytes was shown to bind specifically to a putative peroxisome proliferator response element-one-half-site located at -641/-596 bp. Increasing doses of the ligands troglitazone (1, 10, 20 microM) and fenofibrate (50, 100, 200 microM) but not 15-deoxy-prostaglandin (J(2)) (0.5, 1.0, 2.5 microM) resulted in a significant reduction of both promoter activity and the amount of mRNA expression. Recombinant CORS-26 significantly stimulated the adipocytic secretion of adiponectin and resistin in a dose-dependent manner.DISCUSSION:
The mRNA and protein expression profile puts CORS-26 in the adipocytokine family. Cartonectin is negatively regulated by exogenous, but not endogenous, PPARgamma ligands. Because CORS-26 up-regulates adipokine secretion, it might be involved in metabolic and immunologic pathways.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Alemanha