[Evaluation of strand displacement amplification in the diagnosis of tuberculosis].
Zhonghua Jie He He Hu Xi Za Zhi
; 29(12): 824-7, 2006 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-17327086
ABSTRACT
OBJECTIVE:
To study the clinical significance and reliability of strand displacement amplification (SDA) in detecting Mycobacterium tuberculosis complex.METHODS:
SDA and fluorescence quantitative polymerase chain reaction (FQ-PCR) were employed to detect samples from 453 cases of tuberculosis, including 332 sputum samples, 78 samples of pleural effusion, and 43 samples of cerebrospinal fluid.RESULTS:
In the 332 sputum samples, 131 were culture-positive, of which 110 samples (88 smear-positive) were Mycobacterium tuberculosis complex and 21 samples (20 smear-positive) were nontuberculous Mycobacteria. The sensitivity and specificity of SDA for the 110 samples of Mycobacterium tuberculosis complex and 21 samples of nontuberculous Mycobacteria were 99.1%, 95.2% and 94.6%, 95.2%, respectively. The positive rates in the 311 cases of Mycobacterium tuberculosis complex for SDA and FQ-PCR were 55.3% (172/311) and 47.0% (146/311), respectively. There were 20 smear positive samples in the 121 samples of pleural effusion and cerebrospinal fluid, of which 19 were Mycobacterium tuberculosis, 1 nontuberculous Mycobacterium. The positive rates for SDA and FQ-PCR were 43.4% (52/120) and 33.4% (40/120), respectively. Internal amplification control (IAC) was designed for SDA to achieve accuracy of the results.CONCLUSION:
The automatic assay system of SDA is a rapid and specific test for detecting Mycobacterium tuberculosis complex.
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Base de dados:
MEDLINE
Assunto principal:
Tuberculose
/
Técnicas Bacteriológicas
/
Técnicas de Amplificação de Ácido Nucleico
/
Mycobacterium tuberculosis
Idioma:
Zh
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
China