Nondigest liberation of biomarkers from plasma: a novel two-stage ultrafiltration approach.

Rogatsky, Eduard; Tomuta, Vlad; Stein, Daniel T

Rogatsky, Eduard; Tomuta, Vlad; Stein, Daniel T.

Afiliação

Rogatsky E; Core Analytical Laboratory at General Clinical Research Center, Albert Einstein College of Medicine of Yeshiva University, Bronx, NY 10461, USA. rogatsky@aecom.yu.edu

J Sep Sci ; 30(2): 245-8, 2007 Feb.

Article em En | MEDLINE | ID: mdl-17390619

ABSTRACT

ABSTRACT

A simple procedure for sample preparation of human plasma by two stages of ultrafiltration using one device is described. Our approach is useful for nondigest liberation of biomarkers bound to albumin and other plasma proteins. The analyte contained in the ultrafiltrate can be directly analyzed without additional sample preparation, and quantified by 2-D RP-RP LC/MS.

Assuntos

Proteínas Sanguíneas/análise; Proteínas Sanguíneas/química; Glucagon/sangue; Glucagon/química; Ultrafiltração/métodos; Biomarcadores/sangue; Biomarcadores/química; Humanos; Espectrometria de Massas; Peso Molecular

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Base de dados: MEDLINE Assunto principal: Glucagon / Proteínas Sanguíneas / Ultrafiltração Idioma: En Ano de publicação: 2007 Tipo de documento: Article País de afiliação: Estados Unidos

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