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Involvement of CAPON and nitric oxide synthases in rat muscle regeneration after peripheral nerve injury.
Chen, Mengling; Cheng, Chun; Yan, Meijuan; Niu, Shuqiong; Gao, Shangfeng; Shi, Shuxian; Liu, Haiou; Qin, Yongwei; Shen, Aiguo.
Afiliação
  • Chen M; The Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, Nantong, China.
J Mol Neurosci ; 34(1): 89-100, 2008.
Article em En | MEDLINE | ID: mdl-18157660
ABSTRACT
Carboxy-terminal PDZ ligand of nNOS (CAPON) protein, as an adaptor, binds to nNOS via the PDZ domain helping regulate neuronal nitric oxide synthase (nNOS) activity at post-synaptic sites in neurons (Jaffrey et al., Neuron, 20, 115-124, 1998). Recently, it has been reported that CAPON is present in mouse muscle and may be involved in mouse muscle growth, injury, and repair possibly by regulating the stability, activity, or position of nNOS (Segalat et al., Experimental Cell Research, 302, 170-179, 2005). The present study was to explore the expression patterns and roles of CAPON as well as NOS in rat muscle regeneration after nerve injury. Normal Sprague-Dawley rats were subjected to right sciatic nerve crush injury. Walking track analysis, real time polymerase chain reaction, Western blotting, in situ hybridization, immunocytochemistry, and co-immunoprecipitation techniques were used. It revealed that CAPON mRNA increased, which peaked on days 1 and 28, whereas nNOS mRNA underwent a downregulation in the ipsilateral gastrocnemius muscles after sciatic nerve injury. Their proteins approximately paralleled the mRNA expression. CAPON and nNOS were identified in the activated satellite cells or myotubes and their in vivo interaction was verified. However, eNOS and iNOS proteins suffered an upregulation and were detected in activated satellite cells or myotubes. These data suggest that CAPON and all these three isoforms of NOS might be involved in muscle regeneration after nerve injury. Further study is necessary for a better understanding of the potential functional link between CAPON, NOS, and muscle regeneration, with possible application to therapy for skeletal muscle repair from nerve injury.
Assuntos
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Base de dados: MEDLINE Assunto principal: Regeneração / Músculo Esquelético / Neuropatia Ciática / Proteínas Adaptadoras de Transdução de Sinal / Óxido Nítrico Sintase Tipo I / Traumatismos dos Nervos Periféricos Idioma: En Ano de publicação: 2008 Tipo de documento: Article País de afiliação: China
Buscar no Google
Base de dados: MEDLINE Assunto principal: Regeneração / Músculo Esquelético / Neuropatia Ciática / Proteínas Adaptadoras de Transdução de Sinal / Óxido Nítrico Sintase Tipo I / Traumatismos dos Nervos Periféricos Idioma: En Ano de publicação: 2008 Tipo de documento: Article País de afiliação: China