Restoration of the immunogenicity of cisplatin-induced cancer cell death by endoplasmic reticulum stress.
Oncogene
; 30(10): 1147-58, 2011 Mar 10.
Article
em En
| MEDLINE
| ID: mdl-21151176
ABSTRACT
In contrast to other cytotoxic agents including anthracyclins and oxaliplatin (OXP), cisplatin (CDDP) fails to induce immunogenic tumor cell death that would allow to stimulate an anticancer immune response and hence to amplify its therapeutic efficacy. This failure to induce immunogenic cell death can be attributed to CDDP's incapacity to elicit the translocation of calreticulin (CRT) from the lumen of the endoplasmic reticulum (ER) to the cell surface. Here, we show that, in contrast to OXP, CDDP is unable to activate the protein kinase-like ER kinase (PERK)-dependent phosphorylation of the eukaryotic translation initiation factor 2α (eIF2α). Accordingly, CDDP also failed to stimulate the formation of stress granules and macroautophagy, two processes that only occur after eIF2α phosphorylation. Using a screening method that monitors the voyage of CRT from the ER lumen to the cell surface, we identified thapsigargin (THAPS), an inhibitor of the sarco/ER Ca(2+)-ATPase as a molecule that on its own does not stimulate CRT exposure, yet endows CDDP with the capacity to do so. The combination of ER stress inducers (such as THAPS or tunicamycin) and CDDP effectively induced the translocation of CRT to the plasma membrane, as well as immunogenic cell death, although ER stress or CDDP alone was insufficient to induce CRT exposure and immunogenic cell death. Altogether, our results underscore the contribution of the ER stress response to the immunogenicity of cell death.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteína G de Ligação ao Cálcio S100
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Cisplatino
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Apoptose
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Retículo Endoplasmático
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Neoplasias
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Antineoplásicos
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
França