Retinal pigment epithelial cells upregulate expression of complement factors after co-culture with activated T cells.
Exp Eye Res
; 92(3): 180-8, 2011 Mar.
Article
em En
| MEDLINE
| ID: mdl-21255569
ABSTRACT
In this study we examined the effect of T cell-derived cytokines on retinal pigment epithelial (RPE) cells with respect to expression of complement components. We used an in vitro co-culture system in which CD3/CD28-activated human T cells were separated from the human RPE cell line (ARPE-19) by a membrane. Differential gene expression in the RPE cells of complement factor genes was identified using gene arrays, and selected gene transcripts were validated by q-RT-PCR. Protein expression was determined by ELISA and immunoblotting. Co-culture with activated T cells increased RPE mRNA and/or protein expression of complement components C3, factors B, H, H-like 1, CD46, CD55, CD59, and clusterin, in a dose-dependent manner. Soluble factors derived from activated T cells are capable of increasing expression of complement components in RPE cells. This is important for the further understanding of inflammatory ocular diseases such as uveitis and age-related macular degeneration.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas do Sistema Complemento
/
Ativação Linfocitária
/
Linfócitos T
/
Regulação da Expressão Gênica
/
Epitélio Pigmentado da Retina
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Dinamarca