The low resolution structure of ApoA1 in spherical high density lipoprotein revealed by small angle neutron scattering.
J Biol Chem
; 286(14): 12495-508, 2011 Apr 08.
Article
em En
| MEDLINE
| ID: mdl-21292766
ABSTRACT
Spherical high density lipoprotein (sHDL), a key player in reverse cholesterol transport and the most abundant form of HDL, is associated with cardiovascular diseases. Small angle neutron scattering with contrast variation was used to determine the solution structure of protein and lipid components of reconstituted sHDL. Apolipoprotein A1, the major protein of sHDL, forms a hollow structure that cradles a central compact lipid core. Three apoA1 chains are arranged within the low resolution structure of the protein component as one of three possible global architectures (i) a helical dimer with a hairpin (HdHp), (ii) three hairpins (3Hp), or (iii) an integrated trimer (iT) in which the three apoA1 monomers mutually associate over a portion of the sHDL surface. Cross-linking and mass spectrometry analyses help to discriminate among the three molecular models and are most consistent with the HdHp overall architecture of apoA1 within sHDL.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Apolipoproteína A-I
/
Espalhamento a Baixo Ângulo
/
Lipoproteínas HDL
/
Nêutrons
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Estados Unidos