Locating the barrier for folding of single molecules under an external force.
Phys Rev Lett
; 107(20): 208301, 2011 Nov 11.
Article
em En
| MEDLINE
| ID: mdl-22181779
ABSTRACT
Single-molecule pulling experiments on the folding of biomolecules are usually interpreted with one-dimensional models in which the dynamics occurs on the "pulling coordinate." Paradoxically, the free-energy profile along this coordinate may lack a refolding barrier, yet a barrier is known to exist for folding; thus, it has been argued that pulling experiments do not probe folding. Here, we show that transitions monitored in pulling experiments probe the true folding barrier but that the barrier may be hidden in the projection onto the pulling coordinate. However, one-dimensional theory using the pulling coordinate still yields physically meaningful energy landscape parameters.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
RNA
/
Dobramento de Proteína
/
Fenômenos Mecânicos
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Estados Unidos