Lipopolysaccharide-induced stem cell factor messenger RNA expression and production in odontoblast-like cells.
J Endod
; 38(5): 623-7, 2012 May.
Article
em En
| MEDLINE
| ID: mdl-22515890
ABSTRACT
INTRODUCTION:
This study was done to evaluate the mechanism involved in stem cell factor (SCF) expression and production by lipopolysaccharide (LPS)-stimulated odontoblast (OD)-like cells and to investigate the signal transduction pathway activated in the process.METHODS:
ODs-like cells (MDPC-23) were stimulated with different LPS concentrations for 1, 6, and 24 hours. SCF expression in OD-like cells was analyzed by reverse-transcriptase polymerase chain reaction, and SCF production was assessed by enzyme-linked immunosorbent assay. In another set of experiments, OD-like cells were pretreated with dexamethasone (DEX), MK886 (MK), p42/44 inhibitor (PD 98059 [PD]), p38 inhibitor (SB 202190 [SB]), or PI3K inhibitor (wortmannin [Wort]) for 30 minutes followed by stimulation with LPS (0.1 µg/mL) for 1 hour.RESULTS:
OD-like cells stimulated with LPS (0.1 µg/mL) for 1 hour expressed SCF, but SCF production decreased with increasing LPS concentrations (1, 10, and 100 µg/mL). DEX and MK were able to inhibit SCF messenger RNA (mRNA) expression. PD, SB, and Wort inhibited the SCF mRNA expression.CONCLUSIONS:
LPS-induced SCF mRNA expression and production in OD-like cells occur via leukotriene production or cytokine and/or chemokine formation, activating the p42/44, p38, and PI3K pathways. Data suggest that SCF released by OD-like cells may act as immune response modulators.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
Lipopolissacarídeos
/
Fator de Células-Tronco
/
Odontoblastos
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Brasil