Both CD4⺠and CD8⺠lymphocytes participate in the IFN-γ response to filamentous hemagglutinin from Bordetella pertussis in infants, children, and adults.
Clin Dev Immunol
; 2012: 795958, 2012.
Article
em En
| MEDLINE
| ID: mdl-22550536
ABSTRACT
Infant CD4⺠T-cell responses to bacterial infections or vaccines have been extensively studied, whereas studies on CD8⺠T-cell responses focused mainly on viral and intracellular parasite infections. Here we investigated CD8⺠T-cell responses upon Bordetella pertussis infection in infants, children, and adults and pertussis vaccination in infants. Filamentous hemagglutinin-specific IFN-γ secretion by circulating lymphocytes was blocked by anti-MHC-I or -MHC-II antibodies, suggesting that CD4⺠and CD8⺠T lymphocytes are involved in IFN-γ production. Flow cytometry analyses confirmed that both cell types synthesized antigen-specific IFN-γ, although CD4⺠lymphocytes were the major source of this cytokine. IFN-γ synthesis by CD8⺠cells was CD4⺠T cell dependent, as evidenced by selective depletion experiments. Furthermore, IFN-γ synthesis by CD4⺠cells was sometimes inhibited by CD8⺠lymphocytes, suggesting the presence of CD8⺠regulatory T cells. The role of this dual IFN-γ secretion by CD4⺠and CD8⺠T lymphocytes in pertussis remains to be investigated.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bordetella pertussis
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Linfócitos T CD4-Positivos
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Coqueluche
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Interferon gama
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Linfócitos T CD8-Positivos
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Bélgica