Characterization of an apo-carotenoid 13,14-dioxygenase from Novosphingobium aromaticivorans that converts ß-apo-8'-carotenal to ß-apo-13-carotenone.
Biotechnol Lett
; 34(10): 1851-6, 2012 Oct.
Article
em En
| MEDLINE
| ID: mdl-22711425
ABSTRACT
A putative carotenoid oxygenase from Novosphingobium aromaticivorans was purified with a specific activity of 0.8 U/mg by His-Trap affinity chromatography. The native enzyme was estimated to be a 52 kDa monomer. Enzyme activity for ß-apo-8'-carotenal was maximal at pH 8.0 and 45 °C, with a half life of 15.3 h, K(m) of 21 µM, and k(cat) of 25 l/min. The enzyme exhibited cleavage activity only for carotenoids containing one ß-ionone ring and its catalytic efficiency (k(cat)/K(m)) followed the order ß-apo-8'-carotenal > ß-apo-4'-carotenal > γ-carotene. The enzyme converted these carotenoids to ß-apo-13-carotenones by cleaving their C(13)-C(14) double bonds. The oxygen atom of ß-apo-13-carotenone originated not from water but from molecular oxygen. Thus, the enzyme was an apo-carotenoid 13,14-dioxygenase.
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1
Base de dados:
MEDLINE
Assunto principal:
Oxigenases
/
Carotenoides
/
Sphingomonadaceae
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article