Real-time detection of telomerase activity using the exponential isothermal amplification of telomere repeat assay.
J Am Chem Soc
; 135(5): 1661-4, 2013 Feb 06.
Article
em En
| MEDLINE
| ID: mdl-23186115
ABSTRACT
As crucial pieces in the puzzle of cancer and human aging, telomeres and telomerase are indispensable in modern biology. Here we describe a novel exponential isothermal amplification of telomere repeat (EXPIATR) assay--a sensitive, simple, and reliable in vitro method for measuring telomerase activity in cell extracts. Through a strategically designed path of nucleic acid isothermal amplifications, EXPIATR abandons the expensive thermal cycling protocol and achieves ultrafast detection telomerase activity equivalent to a single HeLa cancer cell can be detected in â¼25 min.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Telômero
/
Telomerase
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Sequências Repetidas Terminais
/
Técnicas de Amplificação de Ácido Nucleico
/
Ensaios Enzimáticos
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos