Longitudinal characterization of functional, morphologic, and biochemical adaptations in mouse skeletal muscle with hindlimb suspension.
Muscle Nerve
; 48(3): 393-402, 2013 Sep.
Article
em En
| MEDLINE
| ID: mdl-23893872
ABSTRACT
INTRODUCTION:
Hindlimb unloading-induced muscle atrophy is often assessed after a homeostatic state is established, thus overlooking the early adaptations that are critical to developing this pattern of atrophy.METHODS:
Muscle function and physiology were characterized at 0, 1, 3, 7, and 14 days of hindlimb suspension (HS).RESULTS:
Reductions in muscle mass were maximal by Day 14 of HS. Functional strength and isolated muscle strength were reduced. MyHC-I and -IIa expressing fibers were reduced in size by Day 7 in the soleus and by Day 14 in the gastrocnemius (MyHC-I fibers only). Atrogin-1 and MuRF1 expression was increased by Day 1 in both the calf and tibialis anterior while IGF-1 expression was significantly reduced on Day 3. Phosphorylation of Akt was reduced on Day 14.CONCLUSIONS:
Insight into these early changes in response to HS improves understanding of the molecular and functional changes that lead to muscle atrophy.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Adaptação Biológica
/
Regulação da Expressão Gênica
/
Músculo Esquelético
/
Elevação dos Membros Posteriores
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos