Mycobacterium bovis BCG killed by extended freeze-drying induces an immunoregulatory profile and protects against atherosclerosis.
J Intern Med
; 275(1): 49-58, 2014 Jan.
Article
em En
| MEDLINE
| ID: mdl-23962000
ABSTRACT
OBJECTIVES:
Atherosclerosis is an inflammatory disease of the arterial wall that leads to myocardial infarction and stroke. Regulatory T cells (Tregs) and IL-10 exert significant anti-atherogenic effects in experimental models of atherosclerosis by modulating vascular inflammation. We have previously shown that Mycobacterium bovis BCG killed by extended freeze-drying (EFD BCG) decreases lung and colon inflammation by recruiting IL-10-producing Tregs. Therefore, the aim of this study was to investigate the effect of EFD BCG on the development of atherosclerosis.DESIGN:
We used two strains of atherosclerosis-prone mice Ldlr(-/-) (four or six EFD BCG injections) and Apoe(-/-) (six injections).RESULTS:
In both models, EFD BCG significantly reduced the size of atherosclerotic lesions, increased IL-10 production and reduced the serum levels of pro-inflammatory cytokines (IL-6, IL-13, KC and tumour necrosis factor-α). Shortly after treatment with EFD BCG, the number of plasmacytoid dendritic cells (pDCs) and Foxp3(+) Tregs in the draining lymph nodes increased. EFD BCG also led to accumulation of Tregs, but not of pDCs in the spleen, and reduced activity of NF-κB and increased activity of PPAR-γ in both the spleen and vascular tissue of treated mice.CONCLUSION:
EFD BCG has atheroprotective effects through IL-10 production and Treg expansion. These findings support a novel approach to the prevention and treatment of atherosclerosis.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Vacina BCG
/
Interleucina-10
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Linfócitos T Reguladores
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Aterosclerose
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Mycobacterium bovis
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Federação Russa