Molecular identification and phylogenetic analysis of Heterakis gallinae from native chickens in Egypt.
Parasitol Res
; 112(10): 3557-60, 2013 Oct.
Article
em En
| MEDLINE
| ID: mdl-23963640
ABSTRACT
The genomic DNA was extracted from nematode parasites, Heterakis gallinae from specific host, native chickens. The polymerase chain reaction (PCR) was applied to make amplification of the DNA content that aids in the further studies. The primer was used in the PCR reactions that have the following sequences forward (5'-GTTTCCGTAGGTGAACCTGC-3') and reverse (5'-ATATGCTTAAGTTCAGCGGGT-3'). It gave total amplified fragment markers at 914 bp. The gene sequences obtained from Egyptian H. gallinae was to make phylogenetic relationships of Egyptian H. gallinae with other nematodes on the database of an accession number (AJ876757), showing that it was nearly similar to Heterakis gallinarum.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Doenças das Aves Domésticas
/
Galinhas
/
Nematoides
/
Infecções por Nematoides
País/Região como assunto:
Africa
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article