One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE).

Brunelle, Julie L; Green, Rachel

Brunelle, Julie L; Green, Rachel.

Afiliação

Brunelle JL; Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Green R; Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: ragreen@jhmi.edu.

Methods Enzymol ; 541: 151-9, 2014.

Article em En | MEDLINE | ID: mdl-24674069

ABSTRACT

ABSTRACT

This protocol describes a denaturing polyacrylamide gel system utilizing sodium dodecyl sulfate (SDS) to separate protein molecules based on size as first described by Laemmli (1970). SDS-PAGE can be used to monitor protein purifications, check the purity of samples, and to estimate molecular weights for unknown proteins.

Assuntos

Eletroforese em Gel de Poliacrilamida/métodos; Proteínas/análise; Eletroforese em Gel de Poliacrilamida/instrumentação; Proteínas/isolamento & purificação

Palavras-chave

Ammonium persulfate (APS); One-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE); Polyacrylamide; Polyacrylamide gel electrophoresis; SDS-PAGE gel; Stacking gel; Tetramethylethylenediamine (TEMED)

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas / Eletroforese em Gel de Poliacrilamida Idioma: En Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Estados Unidos

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