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A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche.
Berns, Nicola; Woichansky, Innokenty; Friedrichsen, Steffen; Kraft, Nadine; Riechmann, Veit.
Afiliação
  • Berns N; Heidelberg University, Medical Faculty Mannheim, Department of Cell and Molecular Biology and German Cancer Research Center (DKFZ), Division of Signaling and Functional Genomics, Ludolf-Krehl-Strasse 13-17, D-68167 Mannheim, Germany.
  • Woichansky I; Heidelberg University, Medical Faculty Mannheim, Department of Cell and Molecular Biology and German Cancer Research Center (DKFZ), Division of Signaling and Functional Genomics, Ludolf-Krehl-Strasse 13-17, D-68167 Mannheim, Germany.
  • Friedrichsen S; Heidelberg University, Medical Faculty Mannheim, Department of Cell and Molecular Biology and German Cancer Research Center (DKFZ), Division of Signaling and Functional Genomics, Ludolf-Krehl-Strasse 13-17, D-68167 Mannheim, Germany.
  • Kraft N; Heidelberg University, Medical Faculty Mannheim, Department of Cell and Molecular Biology and German Cancer Research Center (DKFZ), Division of Signaling and Functional Genomics, Ludolf-Krehl-Strasse 13-17, D-68167 Mannheim, Germany.
  • Riechmann V; Heidelberg University, Medical Faculty Mannheim, Department of Cell and Molecular Biology and German Cancer Research Center (DKFZ), Division of Signaling and Functional Genomics, Ludolf-Krehl-Strasse 13-17, D-68167 Mannheim, Germany veit.riechmann@medma.uni-heidelberg.de.
J Cell Sci ; 127(Pt 12): 2736-48, 2014 Jun 15.
Article em En | MEDLINE | ID: mdl-24762813
The Drosophila oogenesis system provides an excellent model to study the development of epithelial tissues. Here, we report the first genome-scale in vivo RNA interference (RNAi) screen for genes controlling epithelial development. By directly analysing cell and tissue architecture we identified 1125 genes, which we assigned to seven different functions in epithelial formation and homeostasis. We validated the significance of our screen by generating mutants for Vps60, a component of the endosomal sorting complexes required for transport (ESCRT) machinery. This analysis provided new insights into spatiotemporal control of cell proliferation in the follicular epithelium. Previous studies have identified signals controlling divisions in the follicle stem cell niche. However, 99% of cell divisions occur outside of the niche and it is unclear how these divisions are controlled. Our data distinguish two new domains outside of the stem cell niche where there are differing controls on proliferation. One domain abuts the niche and is characterised by ESCRT, Notch and JAK/STAT-mediated control of proliferation. Adjacent to this domain, another domain is defined by loss of the impact of ESCRT on cell division. Thus, during development epithelial cells pass through a variety of microenvironments that exert different modes of proliferation control. The switch between these modes might reflect a decrease in the 'stemness' of epithelial cells over time.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proliferação de Células / Drosophila melanogaster / Células Epiteliais / Nicho de Células-Tronco Idioma: En Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Alemanha

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proliferação de Células / Drosophila melanogaster / Células Epiteliais / Nicho de Células-Tronco Idioma: En Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Alemanha