IgG-mediated anaphylaxis to a synthetic long peptide vaccine containing a B cell epitope can be avoided by slow-release formulation.
J Immunol
; 192(12): 5813-20, 2014 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-24813207
ABSTRACT
Synthetic long peptides (SLP) are a promising vaccine modality to induce therapeutic T cell responses in patients with chronic infections and tumors. We studied different vaccine formulations in mice using SLP derived from carcinoembryonic Ag. We discovered that one of the SLP contains a linear Ab epitope in combination with a CD4 epitope. Repeated vaccination with this carcinoembryonic Ag SLP in mice shows improved T cell responses and simultaneously induced high titers of peptide-specific Abs. These Abs resulted in unexpected anaphylaxis after a third or subsequent vaccinations with the SLP when formulated in saline. Administration of low SLP doses in the slow-release vehicle IFA prevented the anaphylaxis after repeated vaccination. This study underscores both the immunogenicity of SLP vaccination, for inducing T cell as well as B cell responses, and the necessity of safe administration routes.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Peptídeos
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Imunoglobulina G
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Vacinas
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Antígeno Carcinoembrionário
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Epitopos de Linfócito B
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Anafilaxia
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article