In vivo antigen-driven plasmablast enrichment in combination with antigen-specific cell sorting to facilitate the isolation of rare monoclonal antibodies from human B cells.

Lin, Zhonghua; Chiang, Nancy Y; Chai, Ning; Seshasayee, Dhaya; Lee, Wyne P; Balazs, Mercedesz; Nakamura, Gerald; Swem, Lee R

Lin, Zhonghua; Chiang, Nancy Y; Chai, Ning; Seshasayee, Dhaya; Lee, Wyne P; Balazs, Mercedesz; Nakamura, Gerald; Swem, Lee R.

Afiliação

Lin Z; Department of Antibody Engineering, Genentech Inc., South San Francisco, California, USA.
Chiang NY; Department of Antibody Engineering, Genentech Inc., South San Francisco, California, USA.
Chai N; Department of Infectious Diseases, Genentech Inc., South San Francisco, California, USA.
Seshasayee D; Department of Antibody Engineering, Genentech Inc., South San Francisco, California, USA.
Lee WP; Department of Translational Immunology, Genentech Inc., South San Francisco, California, USA.
Balazs M; 1] Department of Translational Immunology, Genentech Inc., South San Francisco, California, USA. [2].
Nakamura G; Department of Antibody Engineering, Genentech Inc., South San Francisco, California, USA.
Swem LR; Department of Infectious Diseases, Genentech Inc., South San Francisco, California, USA.

Nat Protoc ; 9(7): 1563-77, 2014 Jul.

Article em En | MEDLINE | ID: mdl-24901740

Assuntos

Anticorpos Monoclonais/isolamento & purificação; Antígenos/metabolismo; Linfócitos B/metabolismo; Citometria de Fluxo/métodos; Imunoglobulina G/metabolismo; Animais; Anticorpos Antivirais/metabolismo; Antígenos/química; Humanos; Vírus da Influenza A/imunologia; Camundongos SCID

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Imunoglobulina G / Linfócitos B / Citometria de Fluxo / Anticorpos Monoclonais / Antígenos Idioma: En Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Estados Unidos

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