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Decreased lipogenesis in white adipose tissue contributes to the resistance to high fat diet-induced obesity in phosphatidylethanolamine N-methyltransferase-deficient mice.
Gao, Xia; van der Veen, Jelske N; Hermansson, Martin; Ordoñez, Marta; Gomez-Muñoz, Antonio; Vance, Dennis E; Jacobs, René L.
Afiliação
  • Gao X; Group on the Molecular and Cell Biology of Lipids and Department of Biochemistry, University of Alberta, Edmonton, Canada.
  • van der Veen JN; Group on the Molecular and Cell Biology of Lipids and Department of Biochemistry, University of Alberta, Edmonton, Canada.
  • Hermansson M; Group on the Molecular and Cell Biology of Lipids and Department of Biochemistry, University of Alberta, Edmonton, Canada.
  • Ordoñez M; Department of Biochemistry and Molecular Biology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Bilbao, Spain.
  • Gomez-Muñoz A; Department of Biochemistry and Molecular Biology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Bilbao, Spain.
  • Vance DE; Group on the Molecular and Cell Biology of Lipids and Department of Biochemistry, University of Alberta, Edmonton, Canada.
  • Jacobs RL; Group on the Molecular and Cell Biology of Lipids and Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada. Electronic address: rjacobs@ualberta.ca.
Biochim Biophys Acta ; 1851(2): 152-62, 2015 Feb.
Article em En | MEDLINE | ID: mdl-25463480
ABSTRACT
Mice lacking phosphatidylethanolamine N-methyltransferase (PEMT, Pemt(-/-) mice) are resistant to high-fat diet (HFD)-induced obesity (DIO) but develop non-alcoholic steatohepatitis. PEMT expression is strongly induced during differentiation of 3T3-L1 adipocytes. Hence, we hypothesized that white adipose tissue (WAT) might be a key player in the protection against DIO in Pemt(-/-) mice. We fed Pemt(-/-) and Pemt(+/+) mice the HFD for 2 weeks, after which we examined adipocyte differentiation, adipogenesis and lipolysis in WAT. Pemt(-/-) mice gained less body weight, had reduced WAT mass and had smaller adipocytes than Pemt(+/+) mice. The protein levels of adipose differentiation markers FABP4, PPARγ and C/EBPß were not altered by genotype, but acetyl-CoA carboxylase expression and activation was reduced in the Pemt(-/-) mice. The in vivo conversion of [¹4C]acetate to [¹4C]TG in WAT was also lower in Pemt(-/-) mice. The release of glycerol from WAT explants was comparable between Pemt(+/+) and Pemt(-/-) mice under basal condition and in the presence of isoproterenol, indicating unaffected lipolytic capacity. Furthermore, the amounts of leptin, cytokines and chemokines in WAT were not altered by genotype in mice fed the HFD for 2 weeks. However, after 10 weeks of HFD, WAT from Pemt(-/-) mice had dramatically lower leptin, inflammatory cytokines (IL-1 and TNF-α) and chemokines (MCP-1 and RANTES), and significantly higher anti-inflammatory cytokine IL-10 than Pemt(+/+) mice. Together, our data show that PEMT deficiency did not affect the capability for differentiation and lipolysis in WAT. Decreased lipogenesis in WAT may contribute to the resistance to DIO in Pemt(-/-) mice.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fosfatidiletanolamina N-Metiltransferase / Lipogênese / Tecido Adiposo Branco / Dieta Hiperlipídica / Obesidade Idioma: En Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Canadá

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fosfatidiletanolamina N-Metiltransferase / Lipogênese / Tecido Adiposo Branco / Dieta Hiperlipídica / Obesidade Idioma: En Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Canadá