The Fall of a Dogma? Unexpected High T-Cell Memory Response to Staphylococcus aureus in Humans.
J Infect Dis
; 212(5): 830-8, 2015 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-25737563
ABSTRACT
INTRODUCTION:
Though Staphylococcus aureus is a major pathogen, vaccine trials have failed. In contrast, class-switched antibodies specific to S. aureus are common, implying immune memory formation and suggesting a large pool of S. aureus-reactive helper T-cells.OBJECTIVE:
To elucidate the cellular arm of S. aureus-specific immune memory, the T-cell response in humans was characterized.METHODS:
The proliferative response of human peripheral blood mononuclear cells (PBMCs) to S. aureus antigens and the frequency of S. aureus-specific T-cells were quantified by (3)H-thymidine incorporation; cytokine release was measured by flow cytometry.RESULTS:
Staphylococcus aureus particles and extracellular proteins elicited pronounced proliferation in PBMCs of healthy adults. This reflected a memory response with high frequencies of T-cells being activated by single S. aureus antigens. The whole S. aureus-specific T-cell pool was estimated to comprise 3.6% of T-cells with 35-fold differences between individuals (range, 0.2%-5.7%). When exposed to S. aureus antigens, the T-cells released predominantly but not solely T helper (Th)1/Th17 cytokines.CONCLUSIONS:
The large number of S. aureus antigen-reactive memory T-lymphocytes is likely to influence the course of S. aureus infection. To enable rational vaccine design, the naturally acquired human T-cell memory needs to be explored at high priority.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Staphylococcus aureus
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Linfócitos T
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Memória Imunológica
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article