Identification of immediate early response protein 2 as a regulator of angiogenesis through the modulation of endothelial cell motility and adhesion.
Int J Mol Med
; 36(4): 1104-10, 2015 Oct.
Article
em En
| MEDLINE
| ID: mdl-26260137
ABSTRACT
Human immediate early response 2 (IER2) has been characterized as a putative nuclear protein that functions as a transcription factor or transcriptional coactivator in the regulation of cellular responses, and may be involved in the regulation of tumor progression and metastasis. Data from our previous gene expression profile of the human microvascular endothelial cells during capillary morphogenesis showed a significant alteration of IER2 expression, suggesting that IER2 may participate in the regulation of the endothelial cell morphogenesis and angiogenesis. The aim of the present study was to investigate the role of IER2 in cell motility, cellmatrix adhesion and in vitro capillarylike structures formation of the human umbilical vein endothelium cells (HUVECs). IER2 was constitutively expressed in HUVECs, and lentiviralmediated depletion of IER2 significantly reduced the cell motility, cellmatrix adhesion and capillarylike structures formation of HUVECs. Results also showed that depletion and overexpression of IER2 altered the actin cytoskeleton rearrangement in HUVECs. Furthermore, results from western blot analysis showed that the activity of the focal adhesion kinase (FAK) can be regulated by IER2. These results indicated that IER2 regulates endothelial cell motility, adhesion on collagen type I matrix and the capillary tube formation, as the result of the regulation of the actin cytoskeleton rearrangement presumably via a FAKdependent mechanism.
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Base de dados:
MEDLINE
Assunto principal:
Transativadores
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Movimento Celular
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Proteínas Imediatamente Precoces
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Neovascularização Fisiológica
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Células Endoteliais da Veia Umbilical Humana
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article