Inhibition of migration and induction of apoptosis in LoVo human colon cancer cells by polysaccharides from Ganoderma lucidum.
Mol Med Rep
; 12(5): 7629-36, 2015 Nov.
Article
em En
| MEDLINE
| ID: mdl-26397202
ABSTRACT
Ganoderma lucidum polysaccharides (GLPs), which were purified from the medicinal herb G. lucidum followed by ethanol precipitation, protein depletion using the Sevage assay, purification using DEAEcellulose (DE-52), dialysis and the use of ultrafiltration membranes, are used as an ingredient in traditional anticancer treatments in China. The aim of the current study was to evaluate the anticancer effects and investigate the underlying molecular mechanisms of GLPs on LoVo human colon cancer cells. The results demonstrated that the GLPmediated anticancer effect in LoVo cells was characterized by cytotoxicity, migration inhibition, enhanced DNA fragmentation, morphological alterations and increased lactate dehydrogenase release. Furthermore, the activation of caspases3, 8 and 9 was involved in GLPstimulated apoptosis. Additionally, treatment with GLPs promoted the expression of Fas and caspase3 proteins, whilst reducing the expression of cleaved poly(ADPribose) polymerase. These data indicate that GLPs demonstrate potential antitumor activity in human colon cancer cells, predominantly through the inhibition of migration and induction of apoptosis. Furthermore, activation of the Fas/caspase-dependent apoptosis pathway is involved in the cytotoxicity of GLPs.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
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Medicamentos de Ervas Chinesas
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Movimento Celular
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Apoptose
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Neoplasias do Colo
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Reishi
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Antineoplásicos Fitogênicos
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article