Leishmania-FAST15: A rapid, sensitive and low-cost real-time PCR assay for the detection of Leishmania infantum and Leishmania braziliensis kinetoplast DNA in canine blood samples.
Mol Cell Probes
; 31: 65-69, 2017 02.
Article
em En
| MEDLINE
| ID: mdl-27554834
ABSTRACT
We describe an improved real-time PCR assay (designated as "Leishmania-FAST15") for the detection and quantification of Leishmania infantum and Leishmania braziliensis kinetoplast DNA minicircles in canine blood samples. The analytical sensitivity of this technique is 0.1 fg of DNA, which is equivalent to 0.002 parasite per reaction. This assay uses a small reaction volume (15 µl) and is rapid to perform, with the results being available in less than 34 min. This improved assay might also be suitable for detecting and quantifying L. infantum and L. braziliensis DNA in other tissues, such as bone marrow and lymph nodes.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Leishmania braziliensis
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Leishmania infantum
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DNA de Cinetoplasto
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Custos e Análise de Custo
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Cães
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Reação em Cadeia da Polimerase em Tempo Real
País/Região como assunto:
America do sul
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Brasil
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article