Corneal Epithelial Stem Cells Repopulate the Donor Area within 1 Year from Limbus Removal for Limbal Autograft.
Ophthalmology
; 123(12): 2481-2488, 2016 12.
Article
em En
| MEDLINE
| ID: mdl-27665215
ABSTRACT
PURPOSE:
To determine whether limbal epithelial stem cells (LESCs) repopulate the site harvested for limbal autograft transplantation (LAT), the expression of LESCs markers was evaluated in bioptic specimens obtained from the donor area 12 months or more after surgery.DESIGN:
Interventional case series.PARTICIPANTS:
Patients who underwent LAT for unilateral acquired limbal stem cell deficiency after chemical burn.METHODS:
Corneal limbal explants were obtained from 2 sites, the harvested area and the untouched control area, in the donor eyes of 6 patients who previously underwent LAT for unilateral acquired limbal stem cell deficiency after chemical burn. Limbal epithelial stem cells were isolated, and cellular, immunohistochemistry, and histologic parameters were assessed to compare differences between LESCs isolated from harvested or control sites. MAIN OUTCOMEMEASURES:
Presence of LESCs 1 year or more after LAT.RESULTS:
Specific markers (p63, Ki67, K12), percentage of LESCs, cell doubling, and number of passages in culture did not differ significantly between harvested and control sites. However, the distinctive structure of the palisades of Vogt was found only in 2 of 6 harvested sites.CONCLUSIONS:
Limbal epithelial stem cells repopulate the donor site as early as 1 year after limbus removal for LAT. Autologous transplantation of conjunctiva and limbus are safe procedures and can be performed in cases that cannot be treated by simple grafting of LESCs cultured ex vivo.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Limbo da Córnea
/
Epitélio Corneano
/
Transplante de Células-Tronco
/
Sítio Doador de Transplante
/
Reepitelização
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article