Low grade inflammation inhibits VEGF induced HUVECs migration in p53 dependent manner.
Biochem Biophys Res Commun
; 483(2): 803-809, 2017 02 05.
Article
em En
| MEDLINE
| ID: mdl-27998768
ABSTRACT
In the course of studying crosstalk between inflammation and angiogenesis, high doses of pro-inflammatory factors have been reported to induce apoptosis in cells. Under normal circumstances also the pro-inflammatory cytokines are being released in low doses and are actively involved in cell signaling pathways. We studied the effects of low grade inflammation in growth factor induced angiogenesis using tumor necrosis factor alfa (TNFα) and vascular endothelial growth factor A (VEGF) respectively. We found that low dose of TNFα can inhibit VEGF induced angiogenesis in human umbilical vein endothelial cells (HUVECs). Low dose of TNFα induces mild upregulation and moreover nuclear localization of tumor suppressor protein 53 (P53) which causes decrease in inhibitor of DNA binding-1 (Id1) expression and shuttling to the cytoplasm. In absence of Id1, HUVECs fail to upregulate ß3-integrin and cell migration is decreased. Connecting low dose of TNFα induced p53 to ß3-integrin through Id1, we present additional link in cross talk between inflammation and angiogenesis.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteína Supressora de Tumor p53
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Fator A de Crescimento do Endotélio Vascular
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Inflamação
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Japão