Expression analysis of different ß-carotenoid hydroxylase gene families in stress response in Dunaliella viridis.
Yi Chuan
; 39(2): 156-165, 2017 02 20.
Article
em En
| MEDLINE
| ID: mdl-28242602
ABSTRACT
ß-carotenoid hydroxylase (CHYB) is an important rate-limiting enzyme in the biosynthesis of plant carotenoid. In this study, chyb1 and chyb2, two gene families in Dunaliella viridis were obtained by RNA-seq. The fragment of promoters of CHYB family genes, 1 080 bp for chyb1 (GenBank No. KY012338) and 1 155 bp for chyb2 (GenBank No. KY012339) were cloned by the Genome Walking Technology, respectively. Cis-acting elements of two promoters were analyzed by Plantcare soft. The results show that the chyb1 gene promoter contains more cis-acting elements in responses to abiotic stresses, such as methyl jasmonate, arachidonic acid, acetylsalicylic acid, and so on. On the other hand, the chyb2 promoter contains more cis-acting elements in response to light stress. qRT-PCR results show that the mRNA expression levels of CHYBs are modulated by their promoters, and different CHYB gene families response to distinct stresses.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Carotenoides
/
Clorófitas
/
Oxigenases de Função Mista
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
China