Quantifying transcription factor-DNA binding in single cells in vivo with photoactivatable fluorescence correlation spectroscopy.

Zhao, Ziqing Winston; White, Melanie D; Alvarez, Yanina D; Zenker, Jennifer; Bissiere, Stephanie; Plachta, Nicolas

Zhao, Ziqing Winston; White, Melanie D; Alvarez, Yanina D; Zenker, Jennifer; Bissiere, Stephanie; Plachta, Nicolas.

Afiliação

Zhao ZW; Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.
White MD; Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.
Alvarez YD; Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.
Zenker J; Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Bissiere S; Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.
Plachta N; Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.

Nat Protoc ; 12(7): 1458-1471, 2017 Jul.

Article em En | MEDLINE | ID: mdl-28686586

Assuntos

DNA/metabolismo; Análise de Célula Única/métodos; Espectrometria de Fluorescência/métodos; Fatores de Transcrição/metabolismo; Animais; Embrião de Mamíferos; Camundongos; Ligação Proteica; Fatores de Tempo

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espectrometria de Fluorescência / Fatores de Transcrição / DNA / Análise de Célula Única Idioma: En Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Singapura

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