Pseudomonas phage inhibition of Candida albicans.
Microbiology (Reading)
; 163(11): 1568-1577, 2017 Nov.
Article
em En
| MEDLINE
| ID: mdl-28982395
ABSTRACT
Pseudomonas aeruginosa (Pa) and Candida albicans (Ca) are major bacterial and fungal pathogens in immunocompromised hosts, and notably in the airways of cystic fibrosis patients. The bacteriophages of Pa physically alter biofilms, and were recently shown to inhibit the biofilms of Aspergillus fumigatus. To understand the range of this viral-fungal interaction, we studied Pa phages Pf4 and Pf1, and their interactions with Ca biofilm formation and preformed Ca biofilm. Both forms of Ca biofilm development, as well as planktonic Ca growth, were inhibited by either phage. The inhibition of biofilm was reversed by the addition of iron, suggesting that the mechanism of phage action on Ca involves denial of iron. Birefringence studies on added phage showed an ordered structure of binding to Ca. Electron microscopic observations indicated phage aggregation in the biofilm extracellular matrix. Bacteriophage-fungal interactions may be a general feature with several pathogens in the fungal kingdom.
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Base de dados:
MEDLINE
Assunto principal:
Candida albicans
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Fagos de Pseudomonas
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Biofilmes
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Ferro
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Estados Unidos