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Evaluation of the new AspID polymerase chain reaction assay for detection of Aspergillus species: A pilot study.
Prattes, Juergen; Hoenigl, Martin; Zinke, Stefanie E-M; Heldt, Sven; Eigl, Susanne; Johnson, Gemma L; Bustin, Stephen; Stelzl, Evelyn; Kessler, Harald H.
Afiliação
  • Prattes J; Section of Infectious Diseases and Tropical Medicine, Medical University of Graz, Graz, Austria.
  • Hoenigl M; CBmed - Center for Biomarker Research in Medicine, Graz, Austria.
  • Zinke SE; Section of Infectious Diseases and Tropical Medicine, Medical University of Graz, Graz, Austria.
  • Heldt S; CBmed - Center for Biomarker Research in Medicine, Graz, Austria.
  • Eigl S; Division of Pulmonology, Medical University of Graz, Graz, Austria.
  • Johnson GL; Division of Infectious Diseases, University of California San Diego (UCSD), San Diego, CA, USA.
  • Bustin S; Section of Infectious Diseases and Tropical Medicine, Medical University of Graz, Graz, Austria.
  • Stelzl E; Molecular Diagnostics Laboratory & Research Unit Molecular Diagnostics, IHMEM, Medical University of Graz, Graz, Austria.
  • Kessler HH; Section of Infectious Diseases and Tropical Medicine, Medical University of Graz, Graz, Austria.
Mycoses ; 61(6): 355-359, 2018 Jun.
Article em En | MEDLINE | ID: mdl-29460450
ABSTRACT
The newly developed AspID PCR assay for detection of Aspergillus spp. was evaluated with an interlaboratory quality control programme panel and human bronchoalveolar lavage fluid (BALF) samples. With the quality control programme, 8 out of 9 panel members were correctly identified. With the clinical study, 36 BALF samples that had been obtained from 18 patients with invasive pulmonary aspergillosis (IPA) and 18 without IPA were investigated. Sensitivity, specificity, positive and negative likelihood ratio for the AspID assay were 94.1% (95% CI 73.3-99.9), 76.5% (95% CI 50.1-93.2), 4 (95% CI 1.7-9.5) and 0.1 (95% CI 0.01-0.5) respectively.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Aspergillus / Líquido da Lavagem Broncoalveolar / Técnicas de Diagnóstico Molecular / Aspergilose Pulmonar Invasiva / Reação em Cadeia da Polimerase Multiplex Idioma: En Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Áustria
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Aspergillus / Líquido da Lavagem Broncoalveolar / Técnicas de Diagnóstico Molecular / Aspergilose Pulmonar Invasiva / Reação em Cadeia da Polimerase Multiplex Idioma: En Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Áustria