A consensus-guided approach yields a heat-stable alkane-producing enzyme and identifies residues promoting thermostability.

Shakeel, Tabinda; Gupta, Mayank; Fatma, Zia; Kumar, Rakesh; Kumar, Raubins; Singh, Rahul; Sharma, Medha; Jade, Dhananjay; Gupta, Dinesh; Fatma, Tasneem; Yazdani, Syed Shams

Shakeel, Tabinda; Gupta, Mayank; Fatma, Zia; Kumar, Rakesh; Kumar, Raubins; Singh, Rahul; Sharma, Medha; Jade, Dhananjay; Gupta, Dinesh; Fatma, Tasneem; Yazdani, Syed Shams.

Afiliação

Shakeel T; From the Microbial Engineering Group.
Gupta M; DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067 and.
Fatma Z; From the Microbial Engineering Group.
Kumar R; DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067 and.
Kumar R; From the Microbial Engineering Group.
Singh R; DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067 and.
Sharma M; Translational Bioinformatics Group, and.
Jade D; From the Microbial Engineering Group.
Gupta D; From the Microbial Engineering Group.
Fatma T; DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067 and.
Yazdani SS; From the Microbial Engineering Group.

J Biol Chem ; 293(24): 9148-9161, 2018 06 15.

Article em En | MEDLINE | ID: mdl-29632075

Assuntos

Aldeídos/metabolismo; Alcanos/metabolismo; Cianobactérias/enzimologia; Oxigenases/metabolismo; Biocombustíveis/microbiologia; Cianobactérias/química; Cianobactérias/genética; Cianobactérias/metabolismo; Estabilidade Enzimática; Escherichia coli/química; Escherichia coli/enzimologia; Escherichia coli/genética; Escherichia coli/metabolismo; Genes Bacterianos; Fontes Termais/microbiologia; Temperatura Alta; Metagenoma; Modelos Moleculares; Mutagênese Sítio-Dirigida/métodos; Nostoc/química; Nostoc/enzimologia; Nostoc/genética; Nostoc/metabolismo; Oxigenases/química; Oxigenases/genética; Conformação Proteica

Palavras-chave

aldehyde deformylating oxygenase; biofuel; enzyme mechanism; enzyme mutation; long-chain alkane; molecular dynamics; molecular modeling; protein engineering; structurefunction; thermostable enzyme

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oxigenases / Cianobactérias / Aldeídos / Alcanos Idioma: En Ano de publicação: 2018 Tipo de documento: Article

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oxigenases / Cianobactérias / Aldeídos / Alcanos Idioma: En Ano de publicação: 2018 Tipo de documento: Article