DNA:RNA hybrids at telomeres - when it is better to be out of the (R) loop.
FEBS J
; 285(14): 2552-2566, 2018 07.
Article
em En
| MEDLINE
| ID: mdl-29637701
ABSTRACT
R-loops (RLs) are three-stranded nucleic acid structures that contain a DNARNA hybrid and a displaced DNA strand. Genomic regions with GC skew and a G-rich transcript are particularly prone to form RLs. RLs play important physiological roles in cells; however, when present at abnormally high levels, they may threaten genome stability. The perfect GC skew of telomeric repeats and the discovery of telomeric repeat-containing RNA (TERRA), a long noncoding transcript that consists of the G-rich telomeric sequence, make telomeric sequences the perfect candidates for generating RLs. Indeed, in the past 5 years, telomere R-loops (TRLs) have been demonstrated in Saccharomyces cerevisiae, Trypanosoma brucei, and human cells. The presence of TRLs in normal human cells that transcribe low levels of TERRA, suggests a physiological role for these nucleic structures in telomere maintenance. Abnormally enhanced TERRA transcription, as found in several human pathological conditions, leads to high TRL levels and various cellular outcomes, depending on the recombinogenic capabilities of the cells. Study of TRLs in various organisms highlights the necessity for tight regulation of these structures, which can switch from beneficial to detrimental under different conditions. Here, we review the current state of knowledge on TRLs, describe several means by which TRLs are regulated, and discuss how findings from yeast are relevant to human pathological scenarios in which TRLs are deregulated.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Telômero
/
Genoma
/
Face
/
RNA Longo não Codificante
/
Síndromes de Imunodeficiência
/
Hibridização de Ácido Nucleico
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Israel