Tumor Necrosis Factor-α Is Required for Mast Cell-Mediated Host Immunity Against Cutaneous Staphylococcus aureus Infection.
J Infect Dis
; 218(1): 64-74, 2018 06 05.
Article
em En
| MEDLINE
| ID: mdl-29741644
ABSTRACT
Background:
Mast cells (MCs) play a key role in immune process response to invading pathogens.Methods:
This study assessed the involvement of MCs in controlling Staphylococcus aureus infection in a cutaneous infection model of MC-deficient (KitW-sh/W-sh) mice.Results:
KitW-sh/W-sh mice developed significantly larger skin lesions after the cutaneous S. aureus challenge, when compared to wild-type (WT) mice, while MC dysfunction reduced the inflammation response to S. aureus. The levels of tumor necrosis factor (TNF)-α in skin tissues were significantly decreased in KitW-sh/W-sh mice upon infection. Moreover, the exogenous administration of MCs or recombinant TNF-α effectively restored the immune response against S. aureus in KitW-sh/W-sh mice via the recruitment of neutrophils to the infected site. These results indicate that the effects of MC deficiency are largely attributed to the decrease in production of TNF-α in cutaneous S. aureus infection. In addition, S. aureus-induced MC activation was dependent on the c-kit receptor-activated phosphoinositide 3-kinase (PI3K)/AKT/P65-nuclear factor (NF-κB) pathway, which was confirmed by treatment with Masitinib (a c-kit receptor inhibitor), Wortmannin (a PI3K inhibitor), and pyrrolidine dithiocarbamate (a NF-κB inhibitor), respectively.Conclusions:
The present study identifies the critical role of MCs in the host defense against S. aureus infection.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Staphylococcus aureus
/
Infecções Cutâneas Estafilocócicas
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Fator de Necrose Tumoral alfa
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Mastócitos
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China