Characterization of the bagremycin biosynthetic gene cluster in Streptomyces sp. Tü 4128.
Biosci Biotechnol Biochem
; 83(3): 482-489, 2019 Mar.
Article
em En
| MEDLINE
| ID: mdl-30526412
ABSTRACT
Bagremycin A and bagremycin B isolated from Streptomyces sp. Tü 4128 have activities against Gram-positive bacteria, fungi and also have a weak antitumor activity, which make them have great potential for development of novel antibiotics. Here, we report a draft genome 8,424,112 bp in length of S. sp. Tü 4128 by Illumina Hiseq2000, and identify the bagremycins biosynthetic gene cluster (BGC) by bioinformatics analysis. The putative bagremycins BGC includes 16 open reading frames (ORFs) with the functions of biosynthesis, resistance and regulation. Disruptions of relative genes and HPLC analysis of bagremycins production demonstrated that not all the genes within the BGC are responsible for the biosynthesis of bagremycins. In addition, the biosynthetic pathways of bagremycins are proposed for deeper inquiries into their intriguing biosynthetic mechanism.
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Base de dados:
MEDLINE
Assunto principal:
Streptomyces
/
Família Multigênica
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China