Your browser doesn't support javascript.
loading
CiRCus: A Framework to Enable Classification of Complex High-Throughput Experiments.
Seefried, Florian; Schmidt, Tobias; Reinecke, Maria; Heinzlmeir, Stephanie; Kuster, Bernhard; Wilhelm, Mathias.
Afiliação
  • Seefried F; Chair of Proteomics and Bioanalytics , Technical University of Munich , Freising , Germany.
  • Schmidt T; Chair of Proteomics and Bioanalytics , Technical University of Munich , Freising , Germany.
  • Reinecke M; Chair of Proteomics and Bioanalytics , Technical University of Munich , Freising , Germany.
  • Heinzlmeir S; German Cancer Research Center and German Cancer, Consortium , Heidelberg , Germany.
  • Kuster B; Chair of Proteomics and Bioanalytics , Technical University of Munich , Freising , Germany.
  • Wilhelm M; Chair of Proteomics and Bioanalytics , Technical University of Munich , Freising , Germany.
J Proteome Res ; 18(4): 1486-1493, 2019 04 05.
Article em En | MEDLINE | ID: mdl-30799618
ABSTRACT
Despite the increasing use of high-throughput experiments in molecular biology, methods for evaluating and classifying the acquired results have not kept pace, requiring significant manual efforts to do so. Here, we present CiRCus, a framework to generate custom machine learning models to classify results from high-throughput proteomics binding experiments. We show the experimental procedure that guided us to the layout of this framework as well as the usage of the framework on an example data set consisting of 557 166 protein/drug binding curves achieving an AUC of 0.9987. By applying our classifier to the data, only 6% of the data might require manual investigation. CiRCus bundles two applications, a minimal interface to label a training data set (CindeR) and an interface for the generation of random forest classifiers with optional optimization of pretrained models (CurveClassification). CiRCus is available on https//github.com/kusterlab accompanied by an in-depth user manual and video tutorial.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Software / Proteômica / Ensaios de Triagem em Larga Escala / Aprendizado de Máquina Idioma: En Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Alemanha

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Software / Proteômica / Ensaios de Triagem em Larga Escala / Aprendizado de Máquina Idioma: En Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Alemanha