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Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software.
Sage, Daniel; Pham, Thanh-An; Babcock, Hazen; Lukes, Tomas; Pengo, Thomas; Chao, Jerry; Velmurugan, Ramraj; Herbert, Alex; Agrawal, Anurag; Colabrese, Silvia; Wheeler, Ann; Archetti, Anna; Rieger, Bernd; Ober, Raimund; Hagen, Guy M; Sibarita, Jean-Baptiste; Ries, Jonas; Henriques, Ricardo; Unser, Michael; Holden, Seamus.
Afiliação
  • Sage D; Biomedical Imaging Group, School of Engineering, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. daniel.sage@epfl.ch.
  • Pham TA; Biomedical Imaging Group, School of Engineering, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
  • Babcock H; Harvard Center for Advanced Imaging, Harvard University, Cambridge, MA, USA.
  • Lukes T; Laboratory of Nanoscale Biology and Laboratoire d'Optique Biomédicale, STI - IBI, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
  • Pengo T; Department of Radioelectronics, FEE, Czech Technical University, Prague, Czech Republic.
  • Chao J; University of Minnesota Informatics Institute, University of Minnesota Twin Cities, Minneapolis, MN, USA.
  • Velmurugan R; Department of Biomedical Engineering, Texas A&M University, College Station, TX, USA.
  • Herbert A; Department of Molecular and Cellular Medicine, Texas A&M University Health Science Center, College Station, TX, USA.
  • Agrawal A; Department of Molecular and Cellular Medicine, Texas A&M University Health Science Center, College Station, TX, USA.
  • Colabrese S; Department of Microbial Pathogenesis and Immunology, Texas A&M University Health Science Center, Bryan, TX, USA.
  • Wheeler A; MRC Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton, UK.
  • Archetti A; Double Helix LLC, Boulder, CO, USA.
  • Rieger B; Biomedical Imaging Group, School of Engineering, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
  • Ober R; Istituto Italiano di Tecnologia, Genova, Italy.
  • Hagen GM; Advanced Imaging Resource, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, UK.
  • Sibarita JB; Laboratory of Experimental Biophysics, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
  • Ries J; Department of Imaging Physics, Delft University of Technology, Delft, the Netherlands.
  • Henriques R; Department of Biomedical Engineering, Texas A&M University, College Station, TX, USA.
  • Unser M; Department of Molecular and Cellular Medicine, Texas A&M University Health Science Center, College Station, TX, USA.
  • Holden S; Centre for Cancer Immunology, University of Southampton, Southampton, UK.
Nat Methods ; 16(5): 387-395, 2019 05.
Article em En | MEDLINE | ID: mdl-30962624
ABSTRACT
With the widespread uptake of two-dimensional (2D) and three-dimensional (3D) single-molecule localization microscopy (SMLM), a large set of different data analysis packages have been developed to generate super-resolution images. In a large community effort, we designed a competition to extensively characterize and rank the performance of 2D and 3D SMLM software packages. We generated realistic simulated datasets for popular imaging modalities-2D, astigmatic 3D, biplane 3D and double-helix 3D-and evaluated 36 participant packages against these data. This provides the first broad assessment of 3D SMLM software and provides a holistic view of how the latest 2D and 3D SMLM packages perform in realistic conditions. This resource allows researchers to identify optimal analytical software for their experiments, allows 3D SMLM software developers to benchmark new software against the current state of the art, and provides insight into the current limits of the field.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Processamento de Imagem Assistida por Computador / Software / Imageamento Tridimensional / Imagem Individual de Molécula Idioma: En Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Suíça
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Processamento de Imagem Assistida por Computador / Software / Imageamento Tridimensional / Imagem Individual de Molécula Idioma: En Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Suíça