Visualizing Microglia with a Fluorescence Turn-On Ugt1a7c Substrate.
Angew Chem Int Ed Engl
; 58(24): 7972-7976, 2019 06 11.
Article
em En
| MEDLINE
| ID: mdl-31038839
ABSTRACT
Microglia, the brain-resident macrophage, are involved in brain development and contribute to the progression of neural disorders. Despite the importance of microglia, imaging of live microglia at a cellular resolution has been limited to transgenic mice. Efforts have therefore been dedicated to developing new methods for microglia detection and imaging. Using a thorough structure-activity relationships study, we developed CDr20, a high-performance fluorogenic chemical probe that enables the visualization of microglia both inâ
vitro and inâ
vivo. Using a genome-scale CRISPR-Cas9 knockout screen, the UDP-glucuronosyltransferase Ugt1a7c was identified as the target of CDr20. The glucuronidation of CDr20 by Ugt1a7c in microglia produces fluorescence.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Microglia
/
Corantes Fluorescentes
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Singapura