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Best practices and benchmarks for intact protein analysis for top-down mass spectrometry.
Donnelly, Daniel P; Rawlins, Catherine M; DeHart, Caroline J; Fornelli, Luca; Schachner, Luis F; Lin, Ziqing; Lippens, Jennifer L; Aluri, Krishna C; Sarin, Richa; Chen, Bifan; Lantz, Carter; Jung, Wonhyeuk; Johnson, Kendall R; Koller, Antonius; Wolff, Jeremy J; Campuzano, Iain D G; Auclair, Jared R; Ivanov, Alexander R; Whitelegge, Julian P; Pasa-Tolic, Ljiljana; Chamot-Rooke, Julia; Danis, Paul O; Smith, Lloyd M; Tsybin, Yury O; Loo, Joseph A; Ge, Ying; Kelleher, Neil L; Agar, Jeffrey N.
Afiliação
  • Donnelly DP; Barnett Institute of Chemical and Biological Analysis and Departments of Chemistry & Chemical Biology and Pharmaceutical Sciences, Northeastern University, Boston, MA, USA.
  • Rawlins CM; Barnett Institute of Chemical and Biological Analysis and Departments of Chemistry & Chemical Biology and Pharmaceutical Sciences, Northeastern University, Boston, MA, USA.
  • DeHart CJ; Departments of Chemistry and Molecular Biosciences and the Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA.
  • Fornelli L; Departments of Chemistry and Molecular Biosciences and the Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA.
  • Schachner LF; Departments of Chemistry and Molecular Biosciences and the Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA.
  • Lin Z; Department of Cell and Regenerative Biology, Department of Chemistry, Human Proteomics Program, University of Wisconsin-Madison, Madison, WI, USA.
  • Lippens JL; Amgen Research, Discovery Attribute Sciences, Amgen, Thousand Oaks, CA, USA.
  • Aluri KC; Barnett Institute of Chemical and Biological Analysis and Departments of Chemistry & Chemical Biology and Pharmaceutical Sciences, Northeastern University, Boston, MA, USA.
  • Sarin R; Alnylam Pharmaceuticals, Cambridge, MA, USA.
  • Chen B; Barnett Institute of Chemical and Biological Analysis and Departments of Chemistry & Chemical Biology and Pharmaceutical Sciences, Northeastern University, Boston, MA, USA.
  • Lantz C; Biogen, Cambridge, MA, USA.
  • Jung W; Department of Cell and Regenerative Biology, Department of Chemistry, Human Proteomics Program, University of Wisconsin-Madison, Madison, WI, USA.
  • Johnson KR; Department of Chemistry and Biochemistry, Department of Biological Chemistry, and UCLA/DOE Institute of Genomics and Proteomics, University of California, Los Angeles, Los Angeles, CA, USA.
  • Koller A; Department of Chemistry and Biochemistry, Department of Biological Chemistry, and UCLA/DOE Institute of Genomics and Proteomics, University of California, Los Angeles, Los Angeles, CA, USA.
  • Wolff JJ; Barnett Institute of Chemical and Biological Analysis and Departments of Chemistry & Chemical Biology and Pharmaceutical Sciences, Northeastern University, Boston, MA, USA.
  • Campuzano IDG; Barnett Institute of Chemical and Biological Analysis and Departments of Chemistry & Chemical Biology and Pharmaceutical Sciences, Northeastern University, Boston, MA, USA.
  • Auclair JR; Bruker Daltonics, Billerica, MA, USA.
  • Ivanov AR; Amgen Research, Discovery Attribute Sciences, Amgen, Thousand Oaks, CA, USA.
  • Whitelegge JP; Biopharmaceutical Analysis Training Laboratory, Northeastern University, Burlington, MA, USA.
  • Pasa-Tolic L; Barnett Institute of Chemical and Biological Analysis and Departments of Chemistry & Chemical Biology and Pharmaceutical Sciences, Northeastern University, Boston, MA, USA.
  • Chamot-Rooke J; The Pasarow Mass Spectrometry Laboratory, The Jane and Terry Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
  • Danis PO; Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Smith LM; Mass Spectrometry for Biology Unit, Institut Pasteur, USR 2000, CNRS, Paris, France.
  • Tsybin YO; Eastwoods Consulting, Boylston, MA, USA.
  • Loo JA; Department of Chemistry, Genome Center of Wisconsin, University of Wisconsin-Madison, Madison, WI, USA.
  • Ge Y; Spectroswiss, Lausanne, Switzerland.
  • Kelleher NL; Department of Chemistry and Biochemistry, Department of Biological Chemistry, and UCLA/DOE Institute of Genomics and Proteomics, University of California, Los Angeles, Los Angeles, CA, USA.
  • Agar JN; Department of Cell and Regenerative Biology, Department of Chemistry, Human Proteomics Program, University of Wisconsin-Madison, Madison, WI, USA.
Nat Methods ; 16(7): 587-594, 2019 07.
Article em En | MEDLINE | ID: mdl-31249407
ABSTRACT
One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins and proteoforms. Here members of the Consortium for Top-Down Proteomics provide a decision tree that guides researchers to robust protocols for mass analysis of intact proteins (antibodies, membrane proteins and others) from mixtures of varying complexity. We also present cross-platform analytical benchmarks using a protein standard sample, to allow users to gauge their proficiency.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Proteínas / Benchmarking Idioma: En Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Proteínas / Benchmarking Idioma: En Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos