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Transplantation of Human Umbilical Cord Blood-Derived Cellular Fraction Improves Left Ventricular Function and Remodeling After Myocardial Ischemia/Reperfusion.
Zhao, Lin; Cheng, Guangming; Choksi, Kashyap; Samanta, Anweshan; Girgis, Magdy; Soder, Rupal; Vincent, Robert J; Wulser, Michael; De Ruyter, Matt; McEnulty, Patrick; Hauptman, Jeryl; Yang, Yanjuan; Weiner, Carl P; Dawn, Buddhadeb.
Afiliação
  • Zhao L; From the Department of Internal Medicine, University of Nevada, Las Vegas School of Medicine (L.Z., G.C., M.G., J.H., Y.Y., B.D.).
  • Cheng G; From the Department of Internal Medicine, University of Nevada, Las Vegas School of Medicine (L.Z., G.C., M.G., J.H., Y.Y., B.D.).
  • Choksi K; Cardiology Consultants of South Georgia, Thomasville (K.C.).
  • Samanta A; Department of Internal Medicine (A.S.), University of Missouri-Kansas City.
  • Girgis M; From the Department of Internal Medicine, University of Nevada, Las Vegas School of Medicine (L.Z., G.C., M.G., J.H., Y.Y., B.D.).
  • Soder R; Cardiovascular Research Institute, University of Kansas Medical Center, Kansas City (R.S., R.J.V., M.W., C.P.W.).
  • Vincent RJ; Cardiovascular Research Institute, University of Kansas Medical Center, Kansas City (R.S., R.J.V., M.W., C.P.W.).
  • Wulser M; Cardiovascular Research Institute, University of Kansas Medical Center, Kansas City (R.S., R.J.V., M.W., C.P.W.).
  • De Ruyter M; Department of Orthopedic Surgery (M.D.R.), University of Missouri-Kansas City.
  • McEnulty P; Department of Radiology, University of Kansas School of Medicine-Wichita (P.M.).
  • Hauptman J; From the Department of Internal Medicine, University of Nevada, Las Vegas School of Medicine (L.Z., G.C., M.G., J.H., Y.Y., B.D.).
  • Yang Y; From the Department of Internal Medicine, University of Nevada, Las Vegas School of Medicine (L.Z., G.C., M.G., J.H., Y.Y., B.D.).
  • Weiner CP; Cardiovascular Research Institute, University of Kansas Medical Center, Kansas City (R.S., R.J.V., M.W., C.P.W.).
  • Dawn B; From the Department of Internal Medicine, University of Nevada, Las Vegas School of Medicine (L.Z., G.C., M.G., J.H., Y.Y., B.D.).
Circ Res ; 125(8): 759-772, 2019 09 27.
Article em En | MEDLINE | ID: mdl-31462157
ABSTRACT
Rationale Human umbilical cord blood (hUCB) contains diverse populations of stem/progenitor cells. Whether hUCB-derived nonhematopoietic cells would induce cardiac repair remains unknown.

Objective:

To examine whether intramyocardial transplantation of hUCB-derived CD45-Lin- nonhematopoietic cellular fraction after a reperfused myocardial infarction in nonimmunosuppressed rats would improve cardiac function and ameliorate ventricular remodeling. Methods and

Results:

Nonhematopoietic CD45-Lin- cells were isolated from hUCB. Flow cytometry and quantitative polymerase chain reaction were used to characterize this subpopulation. Age-matched male Fischer 344 rats underwent a 30-minute coronary occlusion followed by reperfusion and 48 hours later received intramyocardial injection of vehicle or hUCB CD45-Lin- cells. After 35 days, compared with vehicle-treated rats, CD45-Lin- cell-treated rats exhibited improved left ventricular function, blunted left ventricular hypertrophy, greater preservation of viable myocardium in the infarct zone, and superior left ventricular remodeling. Mechanistically, hUCB CD45-Lin- cell injection favorably modulated molecular pathways regulating myocardial fibrosis, cardiomyocyte apoptosis, angiogenesis, and inflammation in postinfarct ventricular myocardium. Rare persistent transplanted human cells could be detected at both 4 and 35 days after myocardial infarction.

Conclusions:

Transplantation of hUCB-derived CD45-Lin- nonhematopoietic cellular subfraction after a reperfused myocardial infarction in nonimmunosuppressed rats ameliorates left ventricular dysfunction and improves remodeling via favorable paracrine modulation of molecular pathways. These findings with human cells in a clinically relevant model of myocardial ischemia/reperfusion in immunocompetent animals may have significant translational implications.Visual Overview An online visual overview is available for this article.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Traumatismo por Reperfusão Miocárdica / Função Ventricular Esquerda / Remodelação Ventricular / Transplante de Células-Tronco Mesenquimais Idioma: En Ano de publicação: 2019 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Traumatismo por Reperfusão Miocárdica / Função Ventricular Esquerda / Remodelação Ventricular / Transplante de Células-Tronco Mesenquimais Idioma: En Ano de publicação: 2019 Tipo de documento: Article