Integrating enzyme evolution and high-throughput screening for efficient biosynthesis of L-DOPA.

Zeng, Weizhu; Xu, Bingbing; Du, Guocheng; Chen, Jian; Zhou, Jingwen

Zeng, Weizhu; Xu, Bingbing; Du, Guocheng; Chen, Jian; Zhou, Jingwen.

Afiliação

Zeng W; Key Laboratory of Industrial Biotechnology, Ministry of Education and School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.
Xu B; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.
Du G; Key Laboratory of Industrial Biotechnology, Ministry of Education and School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.
Chen J; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.
Zhou J; Jiangsu Provisional Research Center for Bioactive Product Processing Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.

J Ind Microbiol Biotechnol ; 46(12): 1631-1641, 2019 Dec.

Article em En | MEDLINE | ID: mdl-31535250

Assuntos

Levodopa/biossíntese; Tirosina Fenol-Liase/metabolismo; Biocatálise; Reatores Biológicos; Escherichia coli/genética; Escherichia coli/metabolismo

Palavras-chave

Catalytic activity; Error-prone PCR; Fed-batch mode; High-throughput screening; Tyrosine phenol lyase

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Tirosina Fenol-Liase / Levodopa Idioma: En Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China

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