USP3 promotes breast cancer cell proliferation by deubiquitinating KLF5.
J Biol Chem
; 294(47): 17837-17847, 2019 11 22.
Article
em En
| MEDLINE
| ID: mdl-31624151
ABSTRACT
The Krüppel-like factor 5 (KLF5) transcription factor is highly expressed in basal type breast cancer and promotes breast cancer cell proliferation, survival, migration, and tumorigenesis. KLF5 protein stability is regulated by ubiquitination. In this study, ubiquitin-specific protease 3 (USP3) was identified as a new KLF5 deubiquitinase by genome-wide siRNA library screening. We demonstrated that USP3 interacts with KLF5 and stabilizes KLF5 via deubiquitination. USP3 knockdown inhibits breast cancer cell proliferation in vitro and tumorigenesis in vivo, which can be partially rescued by ectopic expression of KLF5. Furthermore, we observed a positive correlation between USP3 and KLF5 protein expression levels in human breast cancer samples. These findings suggest that USP3 is a new KLF5 deubiquitinase and that USP3 may represent a potential therapeutic target for breast cancer.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
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Fatores de Transcrição Kruppel-Like
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Ubiquitinação
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Proteases Específicas de Ubiquitina
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China