Cell-Surface Proteomic Profiling in the Fly Brain Uncovers Wiring Regulators.
Cell
; 180(2): 373-386.e15, 2020 01 23.
Article
em En
| MEDLINE
| ID: mdl-31955847
ABSTRACT
Molecular interactions at the cellular interface mediate organized assembly of single cells into tissues and, thus, govern the development and physiology of multicellular organisms. Here, we developed a cell-type-specific, spatiotemporally resolved approach to profile cell-surface proteomes in intact tissues. Quantitative profiling of cell-surface proteomes of Drosophila olfactory projection neurons (PNs) in pupae and adults revealed global downregulation of wiring molecules and upregulation of synaptic molecules in the transition from developing to mature PNs. A proteome-instructed in vivo screen identified 20 cell-surface molecules regulating neural circuit assembly, many of which belong to evolutionarily conserved protein families not previously linked to neural development. Genetic analysis further revealed that the lipoprotein receptor LRP1 cell-autonomously controls PN dendrite targeting, contributing to the formation of a precise olfactory map. These findings highlight the power of temporally resolved in situ cell-surface proteomic profiling in discovering regulators of brain wiring.
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Base de dados:
MEDLINE
Assunto principal:
Condutos Olfatórios
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Neurônios Receptores Olfatórios
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Proteômica
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article