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Cell-Surface Proteomic Profiling in the Fly Brain Uncovers Wiring Regulators.
Li, Jiefu; Han, Shuo; Li, Hongjie; Udeshi, Namrata D; Svinkina, Tanya; Mani, D R; Xu, Chuanyun; Guajardo, Ricardo; Xie, Qijing; Li, Tongchao; Luginbuhl, David J; Wu, Bing; McLaughlin, Colleen N; Xie, Anthony; Kaewsapsak, Pornchai; Quake, Stephen R; Carr, Steven A; Ting, Alice Y; Luo, Liqun.
Afiliação
  • Li J; Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA. Electronic address: jiefuli@stanford.edu.
  • Han S; Departments of Genetics, Biology, and Chemistry, Chan Zuckerberg Biohub, Stanford University, Stanford, CA 94305, USA.
  • Li H; Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Udeshi ND; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Svinkina T; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Mani DR; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Xu C; Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Guajardo R; Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Xie Q; Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Li T; Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Luginbuhl DJ; Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Wu B; Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • McLaughlin CN; Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Xie A; Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Kaewsapsak P; Departments of Genetics, Biology, and Chemistry, Chan Zuckerberg Biohub, Stanford University, Stanford, CA 94305, USA.
  • Quake SR; Departments of Bioengineering and Applied Physics, Chan Zuckerberg Biohub, Stanford University, Stanford, CA 94305, USA.
  • Carr SA; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Ting AY; Departments of Genetics, Biology, and Chemistry, Chan Zuckerberg Biohub, Stanford University, Stanford, CA 94305, USA. Electronic address: ayting@stanford.edu.
  • Luo L; Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA. Electronic address: lluo@stanford.edu.
Cell ; 180(2): 373-386.e15, 2020 01 23.
Article em En | MEDLINE | ID: mdl-31955847
ABSTRACT
Molecular interactions at the cellular interface mediate organized assembly of single cells into tissues and, thus, govern the development and physiology of multicellular organisms. Here, we developed a cell-type-specific, spatiotemporally resolved approach to profile cell-surface proteomes in intact tissues. Quantitative profiling of cell-surface proteomes of Drosophila olfactory projection neurons (PNs) in pupae and adults revealed global downregulation of wiring molecules and upregulation of synaptic molecules in the transition from developing to mature PNs. A proteome-instructed in vivo screen identified 20 cell-surface molecules regulating neural circuit assembly, many of which belong to evolutionarily conserved protein families not previously linked to neural development. Genetic analysis further revealed that the lipoprotein receptor LRP1 cell-autonomously controls PN dendrite targeting, contributing to the formation of a precise olfactory map. These findings highlight the power of temporally resolved in situ cell-surface proteomic profiling in discovering regulators of brain wiring.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Condutos Olfatórios / Neurônios Receptores Olfatórios / Proteômica Idioma: En Ano de publicação: 2020 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Condutos Olfatórios / Neurônios Receptores Olfatórios / Proteômica Idioma: En Ano de publicação: 2020 Tipo de documento: Article