A Copper (II) Ensemble-Based Fluorescence Chemosensor and Its Application in the 'Naked-Eye' Detection of Biothiols in Human Urine.
Sensors (Basel)
; 20(5)2020 Feb 29.
Article
em En
| MEDLINE
| ID: mdl-32121408
ABSTRACT
Quick and effective detection of biothiols in biological fluids has gained increasing attention due to its vital biological functions. In this paper, a novel reversible fluorescence chemosensor (L-Cu2+) based on a benzocoumarin-Cu2+ ensemble has been developed for the detection of biothiols (Cys, Hcy and GSH) in human urine. The chemosensing ensemble (L-Cu2+) contains a 21 stoichiometry structure between fluorescent ligand L and paramagnetic Cu2+. L was found to exclusively bond with Cu2+ ions accompanied with a dramatic fluorescence quenching maximum at 443 nm and an increase of an absorbance band centered at 378 nm. Then, the in situ generated fluorescence sluggish ensemble, L-Cu2+, was successfully used as a chemosensor for the detection of biothiols with a fluorescence "OFF-ON" response modality. Upon the addition of biothiols, the decomplexation of L-Cu2+ led to the liberation of the fluorescent ligand, L, resulting in the recovery of fluorescence and absorbance spectra. Studies revealed that L-Cu2+ possesses simple synthesis, excellent stability, high sensitivity, reliability at a broad pH range and desired renewability (at least 5 times). The practical application of L-Cu2+ was then demonstrated by the detection of biothiols in human urine sample.
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Base de dados:
MEDLINE
Assunto principal:
Compostos de Sulfidrila
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Técnicas Biossensoriais
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Cobre
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Fluorescência
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China