Compact and reflective light-sheet microscopy for long-term imaging of living embryos.
Appl Opt
; 59(13): D89-D94, 2020 May 01.
Article
em En
| MEDLINE
| ID: mdl-32400629
ABSTRACT
The development of light-sheet fluorescence microscopy has been a revolution for developmental biology as it allows long-term imaging during embryonic development. An important reason behind the quick adoption has been the availability of open hardware alternatives. In this work, we present a robust and compact version of a light-sheet fluorescence microscope that is easy to assemble and requires little to no maintenance. An important aspect of the design is that the illumination unit consists of reflective elements, thereby reducing chromatic aberrations an order of magnitude as compared to refractive counterparts.
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Base de dados:
MEDLINE
Assunto principal:
Drosophila
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Embrião de Mamíferos
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Imagem com Lapso de Tempo
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Imagem Óptica
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Microscopia de Fluorescência
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article