Sensitivity analysis of electrical bioimpedance patterns of breast cancer cells labeled with magnetic nanoparticles: forming the foundation for a biosensor of circulating tumor cells.
Physiol Meas
; 41(6): 064001, 2020 07 01.
Article
em En
| MEDLINE
| ID: mdl-32413882
ABSTRACT
OBJECTIVE:
To determine the sensitivity of electrical impedance spectroscopy (EIS) measurements for the detection of a small concentration of breast cancer cells in suspension, previously labeled with magnetic nanoparticles (MNPs) and separated. Additionally, the relation of electrical impedance to the expression of molecular markers was established.APPROACH:
MCF-7, MDA-MB-231 and SK-BR-3 breast cancer cell lines at different concentrations (50, 500 and 5000 cells/500 µl) were labeled with a magnetic nanoparticle-antibody (MNP-ab) bioconjugate that recognizes the corresponding molecular markers (EpCAM, MUC-1 and HER-2, respectively). Electrical bioimpedance spectra (100 Hz to 1 MHz) were recorded in each case. MAINRESULTS:
At the frequency centered at 100 KHz, EIS displayed a greater sensitivity for magnitude when using 50 cells/500 µl (4.11 ± 0.23, 8.81 ± 1.73 and 17.5 ± 3.61 ohms for MCF-7, MDA-MB-231 and SK-BR-3, respectively). There were no significant differences between the phases of impedance tested at the greatest sensitivity of 1 KHz (-78.05 ± 0.53, -79.23 ± 0.93 and -75.26 ± 0.72 degrees for MCF-7, MDA-MB-231 and SK-BR-3, respectively).SIGNIFICANCE:
Under the present conditions, EIS was sensitive enough to detect a low concentration of breast cancer cells (50 cells/500 µl) and classify the distinct cells lines with a signature impedance pattern, as long as the MNP concentration was very low (⩽0.125 mg MNPs/50 000 cells). The relative expression of the molecular markers on each cancer cell line was related to the magnitude of the electrical impedance.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
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Técnicas Biossensoriais
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Nanopartículas de Magnetita
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Células Neoplásicas Circulantes
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article