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WISP-2 expression induced by Teriparatide treatment affects in vitro osteoblast differentiation and improves in vivo osteogenesis.
Smargiassi, Alberto; Bertacchini, Jessika; Checchi, Marta; Potì, Francesco; Tenedini, Elena; Montosi, Giuliana; Magarò, Maria Sara; Amore, Emanuela; Cavani, Francesco; Ferretti, Marzia; Grisendi, Giulia; Maurel, Delphine B; Palumbo, Carla.
Afiliação
  • Smargiassi A; Indiana Center for Musculoskeletal Health (ICMH), University Building, Indianapolis, IN, USA.
  • Bertacchini J; Department of Biomedical, Metabolic and Neural Sciences, Section of Human Morphology, University of Modena and Reggio Emilia, Modena, Italy. Electronic address: jbertacchini@unimore.it.
  • Checchi M; Department of Biomedical, Metabolic and Neural Sciences, Section of Human Morphology, University of Modena and Reggio Emilia, Modena, Italy.
  • Potì F; Department of Medicine and Surgery, Unit of Neurosciences, University of Parma, Parma, Italy.
  • Tenedini E; Center for Genome Research, Department of Medical and Surgical Sciences, University of Modena and Reggio Emilia, Modena, Italy.
  • Montosi G; Center for Hemochromatosis, Department of Internal Medicine II, University of Modena and Reggio Emilia, Modena, Italy.
  • Magarò MS; Department of Biomedical, Metabolic and Neural Sciences, Section of Human Morphology, University of Modena and Reggio Emilia, Modena, Italy.
  • Amore E; Department of Biomedical, Metabolic and Neural Sciences, Section of Human Morphology, University of Modena and Reggio Emilia, Modena, Italy.
  • Cavani F; Department of Biomedical, Metabolic and Neural Sciences, Section of Human Morphology, University of Modena and Reggio Emilia, Modena, Italy.
  • Ferretti M; Department of Biomedical, Metabolic and Neural Sciences, Section of Human Morphology, University of Modena and Reggio Emilia, Modena, Italy.
  • Grisendi G; Laboratory of Experimental Epileptology, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Modena, Italy.
  • Maurel DB; Pharmaceutical Sciences Department, University of Bordeaux, BioTis, INSERM Unit 1026, Bordeaux, France.
  • Palumbo C; Department of Biomedical, Metabolic and Neural Sciences, Section of Human Morphology, University of Modena and Reggio Emilia, Modena, Italy.
Mol Cell Endocrinol ; 513: 110817, 2020 08 01.
Article em En | MEDLINE | ID: mdl-32439416
The Osteocyte, recognized as a major orchestrator of osteoblast and osteoclast activity, is the most important key player during bone remodeling processes. Imbalances occurring during bone remodeling, caused by hormone perturbations or by mechanical loading alterations, can induce bone pathologies such as osteoporosis. Recently, the active fraction of parathormone, PTH (1-34) or Teriparatide (TPTD), was chosen as election treatment for osteoporosis. The effect of such therapy is dependent on the temporal manner of administration. The molecular reasons why the type of administration regimen is so critical for the fate of bone remodeling are numerous and not yet well known. Our study attempts to analyze diverse signaling pathways directly activated in osteocytes upon TPTD treatment. By means of gene array analysis, we found many molecules upregulated or downregulated in osteocytes. Later, we paid attention to Wisp-2, a protein involved in the Wnt pathway, that is secreted by MLO-Y4 cells and increases upon TPTD treatment and that is able to positively influence the early phases of osteogenic differentiation. We also confirmed the pro osteogenic property of Wisp-2 during mesenchymal stem cell differentiation into the preliminary osteoblast phenotype. The same results were confirmed with an in vivo approach confirming a remarkable Wisp-2 expression in metaphyseal trabecular bone. These results highlighted the anabolic roles unrolled by osteocytes in controlling the action of neighboring cells, suggesting that the perturbation of certain signaling cascades, such as the Wnt pathway, is crucial for the positive regulation of bone formation.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Osteoblastos / Teriparatida / Peptídeos e Proteínas de Sinalização Intracelular Idioma: En Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Osteoblastos / Teriparatida / Peptídeos e Proteínas de Sinalização Intracelular Idioma: En Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Estados Unidos